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        Download the raw data used to create the plots in this report below:

        Note that additional data was saved in WGS-Analysis-Report_multiqc_report_data when this report was generated.


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        If you use plots from MultiQC in a publication or presentation, please cite:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

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        Please remember to cite the tools that you use in your analysis.

        To help with this, you can download publication details of the tools mentioned in this report:

        About MultiQC

        This report was generated using MultiQC, version 1.21

        You can see a YouTube video describing how to use MultiQC reports here: https://youtu.be/qPbIlO_KWN0

        For more information about MultiQC, including other videos and extensive documentation, please visit http://multiqc.info

        You can report bugs, suggest improvements and find the source code for MultiQC on GitHub: https://github.com/MultiQC/MultiQC

        MultiQC is published in Bioinformatics:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

        WGS Analysis Report

        This report includes summaries of data quality, data processing, and snapshots of results for your WGS study. This report should assist you to get a general picture of the study, to spot any irregularities in the sample or data, and to explore the most significant results.

        Please consult our WGS report documentation on how to use this report.
        Not sure where to start?   Watch a tutorial video   (6:06)

        General Statistics

        Showing 5/5 rows and 18/35 columns.
        Sample Name
        ≥ 30X
        Median
        Change rate
        Ts/Tv
        M Variants
        Vars
        SNP
        Indel
        Ts/Tv
        Duplication
        Error rate
        Non-primary
        Reads mapped
        % Mapped
        % Proper pairs
        Total seqs
        GC content
        % Adapter
        in4276_1
        93.0%
        63.0X
        426
        1.980
        7.24M
        7138307
        5575275
        1569067
        1.12
        13.0%
        0.76%
        0.0M
        1467.8M
        99.9%
        99.1%
        1468.8M
        40.8%
        4.7%
        in4276_2
        91.0%
        46.0X
        406
        1.992
        7.60M
        7499329
        5762463
        1742280
        1.07
        12.3%
        0.52%
        0.0M
        1106.0M
        99.7%
        90.3%
        1109.7M
        41.4%
        16.2%
        in4276_3
        85.0%
        38.0X
        358
        1.998
        8.61M
        8510434
        6175965
        2339307
        0.96
        11.6%
        0.56%
        0.0M
        905.8M
        99.8%
        82.7%
        908.1M
        40.6%
        19.8%
        in4276_4
        93.0%
        49.0X
        440
        1.990
        7.01M
        6895279
        5398664
        1501801
        1.22
        14.3%
        0.49%
        0.0M
        1165.2M
        99.9%
        98.4%
        1166.2M
        40.7%
        9.7%
        in4276_5
        93.0%
        53.0X
        416
        1.994
        7.42M
        7307703
        5539370
        1773113
        1.18
        12.5%
        0.48%
        0.0M
        1429.7M
        99.9%
        98.8%
        1431.0M
        40.6%
        47.6%

        Mosdepth

        Mosdepth performs fast BAM/CRAM depth calculation for WGS, exome, or targeted sequencing.DOI: 10.1093/bioinformatics/btx699.

        Cumulative coverage distribution

        Proportion of bases in the reference genome with, at least, a given depth of coverage. Note that for 5 samples, a BED file was provided, so the data was calculated across those regions. For 5 samples, it's calculated across the entire genome length. 5 samples have both global and region reports, and we are showing the data for regions

        For a set of DNA or RNA reads mapped to a reference sequence, such as a genome or transcriptome, the depth of coverage at a given base position is the number of high-quality reads that map to the reference at that position, while the breadth of coverage is the fraction of the reference sequence to which reads have been mapped with at least a given depth of coverage (Sims et al. 2014).

        Defining coverage breadth in terms of coverage depth is useful, because sequencing experiments typically require a specific minimum depth of coverage over the region of interest (Sims et al. 2014), so the extent of the reference sequence that is amenable to analysis is constrained to lie within regions that have sufficient depth. With inadequate sequencing breadth, it can be difficult to distinguish the absence of a biological feature (such as a gene) from a lack of data (Green 2007).

        For increasing coverage depths (1×, 2×, …, N×), coverage breadth is calculated as the percentage of the reference sequence that is covered by at least that number of reads, then plots coverage breadth (y-axis) against coverage depth (x-axis). This plot shows the relationship between sequencing depth and breadth for each read dataset, which can be used to gauge, for example, the likely effect of a minimum depth filter on the fraction of a genome available for analysis.

        0X10X20X30X40X50X60X70X80X0%20%40%60%80%100%
        Mosdepth: Cumulative coverage distributionCumulative Coverage (X)% bases in genome/regions covered by at least X reads
        Created with MultiQC

        Coverage distribution

        Proportion of bases in the reference genome with a given depth of coverage. Note that for 5 samples, a BED file was provided, so the data was calculated across those regions. For 5 samples, it's calculated across the entire genome length. 5 samples have both global and region reports, and we are showing the data for regions

        For a set of DNA or RNA reads mapped to a reference sequence, such as a genome or transcriptome, the depth of coverage at a given base position is the number of high-quality reads that map to the reference at that position (Sims et al. 2014).

        Bases of a reference sequence (y-axis) are groupped by their depth of coverage (0×, 1×, …, N×) (x-axis). This plot shows the frequency of coverage depths relative to the reference sequence for each read dataset, which provides an indirect measure of the level and variation of coverage depth in the corresponding sequenced sample.

        If reads are randomly distributed across the reference sequence, this plot should resemble a Poisson distribution (Lander & Waterman 1988), with a peak indicating approximate depth of coverage, and more uniform coverage depth being reflected in a narrower spread. The optimal level of coverage depth depends on the aims of the experiment, though it should at minimum be sufficiently high to adequately address the biological question; greater uniformity of coverage is generally desirable, because it increases breadth of coverage for a given depth of coverage, allowing equivalent results to be achieved at a lower sequencing depth (Sampson et al. 2011; Sims et al. 2014). However, it is difficult to achieve uniform coverage depth in practice, due to biases introduced during sample preparation (van Dijk et al. 2014), sequencing (Ross et al. 2013) and read mapping (Sims et al. 2014).

        This plot may include a small peak for regions of the reference sequence with zero depth of coverage. Such regions may be absent from the given sample (due to a deletion or structural rearrangement), present in the sample but not successfully sequenced (due to bias in sequencing or preparation), or sequenced but not successfully mapped to the reference (due to the choice of mapping algorithm, the presence of repeat sequences, or mismatches caused by variants or sequencing errors). Related factors cause most datasets to contain some unmapped reads (Sims et al. 2014).

        0X10X20X30X40X50X60X70X80X0%1%2%3%4%5%6%
        Mosdepth: Coverage distributionCoverage (X)% bases in genome/regions covered by X reads
        Created with MultiQC

        Average coverage per contig

        Average coverage per contig or chromosome

        chr1chr4chr7chr10chr13chr16chr19chr22chrMchr1_KI270708v1_randomchr1_KI270711v1_randomchr1_KI270714v1_randomchr3_GL000221v1_randomchr9_KI270717v1_randomchr9_KI270720v1_randomchr14_KI270722v1_randomchr14_KI270724v1_randomchr15_KI270727v1_randomchr17_KI270729v1_randomchr22_KI270732v1_randomchr22_KI270736v1_randomchr22_KI270739v1_randomchrUn_KI270303v1chrUn_KI270320v1chrUn_KI270315v1chrUn_KI270317v1chrUn_KI270414v1chrUn_KI270420v1chrUn_KI270422v1chrUn_KI270429v1chrUn_KI270465v1chrUn_KI270438v1chrUn_KI270509v1chrUn_KI270516v1chrUn_KI270522v1chrUn_KI270507v1chrUn_KI270528v1chrUn_KI270538v1chrUn_KI270583v1chrUn_KI270581v1chrUn_KI270590v1chrUn_KI270588v1chrUn_KI270330v1chrUn_KI270333v1chrUn_KI270340v1chrUn_KI270363v1chrUn_KI270366v1chrUn_KI270389v1chrUn_KI270395v1chrUn_KI270394v1chrUn_KI270383v1chrUn_KI270392v1chrUn_KI270382v1chrUn_KI270372v1chrUn_KI270371v1chrUn_GL000195v1chrUn_GL000224v1chrUn_GL000213v1chrUn_KI270745v1chrUn_KI270748v1chrUn_KI270751v1chrUn_KI270755v1chrUn_GL000214v1chrUn_GL000218v1KCNJ18_chr17_22629421_22688415KMT2C_chr13_14464876_14502703KMT2C_chr13_10744786_10790087KMT2C_chr13_14408161_14474907KMT2C_chr15_5960377_6027329KMT2C_chr21_7659652_7674726KMT2C_chr22_6626735_6693166KMT2C_chr22_11157169_111898150x100x200x300x
        Mosdepth: Coverage per contigRegionAverage Coverage
        Created with MultiQC

        XY coverage

        0%20%40%60%80%100%in4276_5in4276_4in4276_3in4276_2in4276_1
        Chromosome XChromosome YMosdepth: chrXY coveragePercent of X+Y coverage
        Created with MultiQC

        SNPeff

        SNPeff is a genetic variant annotation and effect prediction toolbox. It annotates and predicts the effects of variants on genes (such as amino acid changes). .DOI: 10.4161/fly.19695.

        Variants by Genomic Region

        The stacked bar plot shows locations of detected variants in the genome and the number of variants for each location.

        The upstream and downstream interval size to detect these genomic regions is 5000bp by default.

        02M4M6M8M10Min4276_5in4276_4in4276_3in4276_2in4276_1
        IntronIntergenicTranscriptUpstreamDownstreamExonUTR 3 PrimeUTR 5 PrimeSplice Site RegionSplice Site AcceptorSplice Site DonorGeneSnpEff: Counts by Genomic Region# Reads
        Created with MultiQC

        Variant Effects by Impact

        The stacked bar plot shows the putative impact of detected variants and the number of variants for each impact.

        There are four levels of impacts predicted by SnpEff:

        • High: High impact (like stop codon)
        • Moderate: Middle impact (like same type of amino acid substitution)
        • Low: Low impact (ie silence mutation)
        • Modifier: No impact
        02M4M6M8M10Min4276_5in4276_4in4276_3in4276_2in4276_1
        ModifierLowModerateHighSnpEff: Counts by Effects Impact# Reads
        Created with MultiQC

        Variants by Effect Types

        The stacked bar plot shows the effect of variants at protein level and the number of variants for each effect type.

        This plot shows the effect of variants with respect to the mRNA.

        02M4M6M8M10Min4276_5in4276_4in4276_3in4276_2in4276_1
        Intron VariantIntergenic RegionIntragenic VariantUpstream Gene VariantDownstream Gene VariantNon Coding Transcript Exon Variant3 Prime UTR VariantMissense VariantSynonymous Variant5 Prime UTR VariantSplice Region VariantFrameshift Variant5 Prime UTR Premature Start Codon Gain VariantSplice Acceptor VariantSplice Donor VariantDisruptive Inframe DeletionStop GainedDisruptive Inframe InsertionConservative Inframe DeletionConservative Inframe InsertionStart LostInitiator Codon VariantStop LostNon Coding Transcript VariantBidirectional Gene FusionGene FusionStop Retained VariantStart Retained VariantTranscript Ablation5 Prime UTR TruncationExon Loss VariantSnpEff: Counts by Effect Types# Reads
        Created with MultiQC

        Variants by Functional Class

        The stacked bar plot shows the effect of variants and the number of variants for each effect type.

        This plot shows the effect of variants on the translation of the mRNA as protein. There are three possible cases:

        • Silent: The amino acid does not change.
        • Missense: The amino acid is different.
        • Nonsense: The variant generates a stop codon.
        05k10k15k20k25k30k35kin4276_5in4276_4in4276_3in4276_2in4276_1
        MissenseSilentNonsenseSnpEff: Counts by Functional Class# Reads
        Created with MultiQC

        Variant Qualities

        The line plot shows the quantity as function of the variant quality score.

        The quality score corresponds to the QUAL column of the VCF file. This score is set by the variant caller.

        010203040506000.5M1M1.5M2M2.5M3M3.5M
        SnpEff: QualitiesValuesCount
        Created with MultiQC

        Bcftools

        Bcftools contains utilities for variant calling and manipulating VCFs and BCFs.DOI: 10.1093/gigascience/giab008.

        Variant Substitution Types

        01M2M3M4M5M6Min4276_5in4276_4in4276_3in4276_2in4276_1
        A>CA>GA>TC>AC>GC>TG>AG>CG>TT>AT>CT>GBcftools Stats: Substitutions# Substitutions
        Created with MultiQC

        Variant Quality

        0102030405060708000.5M1M1.5M2M
        Bcftools Stats: Variant Quality CountQualityCount
        Created with MultiQC

        Indel Distribution

        −60−40−2002040600100k200k300k400k500k600k700k
        Bcftools Stats: Indel DistributionInDel Length (bp)Count
        Created with MultiQC

        GATK4 MarkDuplicates

        GATK4 MarkDuplicates metrics generated either by GATK4 MarkDuplicates or EstimateLibraryComplexity (with --use_gatk_spark).

        Mark Duplicates

        Number of reads, categorised by duplication state. Pair counts are doubled - see help text for details.

        The table in the Picard metrics file contains some columns referring read pairs and some referring to single reads.

        To make the numbers in this plot sum correctly, values referring to pairs are doubled according to the scheme below:

        • READS_IN_DUPLICATE_PAIRS = 2 * READ_PAIR_DUPLICATES
        • READS_IN_UNIQUE_PAIRS = 2 * (READ_PAIRS_EXAMINED - READ_PAIR_DUPLICATES)
        • READS_IN_UNIQUE_UNPAIRED = UNPAIRED_READS_EXAMINED - UNPAIRED_READ_DUPLICATES
        • READS_IN_DUPLICATE_PAIRS_OPTICAL = 2 * READ_PAIR_OPTICAL_DUPLICATES
        • READS_IN_DUPLICATE_PAIRS_NONOPTICAL = READS_IN_DUPLICATE_PAIRS - READS_IN_DUPLICATE_PAIRS_OPTICAL
        • READS_IN_DUPLICATE_UNPAIRED = UNPAIRED_READ_DUPLICATES
        • READS_UNMAPPED = UNMAPPED_READS
        0%20%40%60%80%100%in4276_5in4276_4in4276_3in4276_2in4276_1
        Unique PairsUnique UnpairedDuplicate Pairs OpticalDuplicate Pairs NonopticalDuplicate UnpairedUnmappedGATK4 MarkDuplicates: Deduplication Stats# Reads
        Created with MultiQC

        Samtools Flagstat

        Samtools is a suite of programs for interacting with high-throughput sequencing data.DOI: 10.1093/bioinformatics/btp352.

        Percent mapped

        Alignment metrics from samtools stats; mapped vs. unmapped reads vs. reads mapped with MQ0.

        For a set of samples that have come from the same multiplexed library, similar numbers of reads for each sample are expected. Large differences in numbers might indicate issues during the library preparation process. Whilst large differences in read numbers may be controlled for in downstream processings (e.g. read count normalisation), you may wish to consider whether the read depths achieved have fallen below recommended levels depending on the applications.

        Low alignment rates could indicate contamination of samples (e.g. adapter sequences), low sequencing quality or other artefacts. These can be further investigated in the sequence level QC (e.g. from FastQC).

        Reads mapped with MQ0 often indicate that the reads are ambiguously mapped to multiple locations in the reference sequence. This can be due to repetitive regions in the genome, the presence of alternative contigs in the reference, or due to reads that are too short to be uniquely mapped. These reads are often filtered out in downstream analyses.

        00.2B0.4B0.6B0.8B1B1.2B1.4Bin4276_5in4276_4in4276_3in4276_2in4276_1
        Mapped (with MQ>0)MQ0UnmappedSamtools stats: Alignment Scores# Reads
        Created with MultiQC

        Alignment stats

        This module parses the output from samtools stats. All numbers in millions.

        0M200M400M600M800M1000M1200M1400MTotal sequences 0M200M400M600M800M1000M1200M1400MMapped & paired 0M200M400M600M800M1000M1200M1400MProperly paired 0M200M400M600M800M1000M1200M1400MDuplicated 0M200M400M600M800M1000M1200M1400MQC Failed 0M200M400M600M800M1000M1200M1400MReads MQ0 0Mb50kMb100kMb150kMb200kMbMapped bases (CIGAR) 0Mb50kMb100kMb150kMb200kMbBases Trimmed 0Mb50kMb100kMb150kMb200kMbDuplicated bases 0M200M400M600M800M1000M1200M1400MDiff chromosomes 0M200M400M600M800M1000M1200M1400MOther orientation 0M200M400M600M800M1000M1200M1400MInward pairs 0M200M400M600M800M1000M1200M1400MOutward pairs
        Samtools stats: Alignment Stats
        Created with MultiQC

        FastQC (raw)

        FastQC (raw) is a quality control tool for high throughput sequence data, written by Simon Andrews at the Babraham Institute in Cambridge.

        Sequence Counts

        Sequence counts for each sample. Duplicate read counts are an estimate only.

        This plot show the total number of reads, broken down into unique and duplicate if possible (only more recent versions of FastQC give duplicate info).

        You can read more about duplicate calculation in the FastQC documentation. A small part has been copied here for convenience:

        Only sequences which first appear in the first 100,000 sequences in each file are analysed. This should be enough to get a good impression for the duplication levels in the whole file. Each sequence is tracked to the end of the file to give a representative count of the overall duplication level.

        The duplication detection requires an exact sequence match over the whole length of the sequence. Any reads over 75bp in length are truncated to 50bp for this analysis.

        0100M200M300M400M500M600M700Min4276_5in4276_4in4276_3in4276_2in4276_1
        Unique ReadsDuplicate ReadsFastQC: Sequence CountsNumber of reads
        Created with MultiQC

        Sequence Quality Histograms
        5
        0
        0

        The mean quality value across each base position in the read.

        To enable multiple samples to be plotted on the same graph, only the mean quality scores are plotted (unlike the box plots seen in FastQC reports).

        Taken from the FastQC help:

        The y-axis on the graph shows the quality scores. The higher the score, the better the base call. The background of the graph divides the y axis into very good quality calls (green), calls of reasonable quality (orange), and calls of poor quality (red). The quality of calls on most platforms will degrade as the run progresses, so it is common to see base calls falling into the orange area towards the end of a read.

        02040608010012014005101520253035
        FastQC: Mean Quality ScoresPosition (bp)Phred Score
        Created with MultiQC

        Per Sequence Quality Scores
        5
        0
        0

        The number of reads with average quality scores. Shows if a subset of reads has poor quality.

        From the FastQC help:

        The per sequence quality score report allows you to see if a subset of your sequences have universally low quality values. It is often the case that a subset of sequences will have universally poor quality, however these should represent only a small percentage of the total sequences.

        051015202530350 reads50M reads100M reads150M reads200M reads250M reads300M reads350M reads400M reads
        FastQC: Per Sequence Quality ScoresMean Sequence Quality (Phred Score)Count
        Created with MultiQC

        Per Base Sequence Content
        1
        2
        2

        The proportion of each base position for which each of the four normal DNA bases has been called.

        To enable multiple samples to be shown in a single plot, the base composition data is shown as a heatmap. The colours represent the balance between the four bases: an even distribution should give an even muddy brown colour. Hover over the plot to see the percentage of the four bases under the cursor.

        To see the data as a line plot, as in the original FastQC graph, click on a sample track.

        From the FastQC help:

        Per Base Sequence Content plots out the proportion of each base position in a file for which each of the four normal DNA bases has been called.

        In a random library you would expect that there would be little to no difference between the different bases of a sequence run, so the lines in this plot should run parallel with each other. The relative amount of each base should reflect the overall amount of these bases in your genome, but in any case they should not be hugely imbalanced from each other.

        It's worth noting that some types of library will always produce biased sequence composition, normally at the start of the read. Libraries produced by priming using random hexamers (including nearly all RNA-Seq libraries) and those which were fragmented using transposases inherit an intrinsic bias in the positions at which reads start. This bias does not concern an absolute sequence, but instead provides enrichement of a number of different K-mers at the 5' end of the reads. Whilst this is a true technical bias, it isn't something which can be corrected by trimming and in most cases doesn't seem to adversely affect the downstream analysis.

        Click a sample row to see a line plot for that dataset.
        Rollover for sample name
        Position: -
        %T: -
        %C: -
        %A: -
        %G: -

        Per Sequence GC Content
        0
        5
        0

        The average GC content of reads. Normal random library typically have a roughly normal distribution of GC content.

        From the FastQC help:

        This module measures the GC content across the whole length of each sequence in a file and compares it to a modelled normal distribution of GC content.

        In a normal random library you would expect to see a roughly normal distribution of GC content where the central peak corresponds to the overall GC content of the underlying genome. Since we don't know the the GC content of the genome the modal GC content is calculated from the observed data and used to build a reference distribution.

        An unusually shaped distribution could indicate a contaminated library or some other kinds of biased subset. A normal distribution which is shifted indicates some systematic bias which is independent of base position. If there is a systematic bias which creates a shifted normal distribution then this won't be flagged as an error by the module since it doesn't know what your genome's GC content should be.

        0% GC20% GC40% GC60% GC80% GC100% GC0%0.5%1%1.5%2%2.5%3%3.5%4%
        FastQC: Per Sequence GC Content% GCPercentage
        Created with MultiQC

        Per Base N Content
        5
        0
        0

        The percentage of base calls at each position for which an N was called.

        From the FastQC help:

        If a sequencer is unable to make a base call with sufficient confidence then it will normally substitute an N rather than a conventional base call. This graph shows the percentage of base calls at each position for which an N was called.

        It's not unusual to see a very low proportion of Ns appearing in a sequence, especially nearer the end of a sequence. However, if this proportion rises above a few percent it suggests that the analysis pipeline was unable to interpret the data well enough to make valid base calls.

        0204060801001201400%20%40%60%80%100%
        FastQC: Per Base N ContentPosition in Read (bp)Percentage N-Count
        Created with MultiQC

        Sequence Length Distribution
        5
        0
        0

        All samples have sequences of a single length (151bp).

        Sequence Duplication Levels
        5
        0
        0

        The relative level of duplication found for every sequence.

        From the FastQC Help:

        In a diverse library most sequences will occur only once in the final set. A low level of duplication may indicate a very high level of coverage of the target sequence, but a high level of duplication is more likely to indicate some kind of enrichment bias (eg PCR over amplification). This graph shows the degree of duplication for every sequence in a library: the relative number of sequences with different degrees of duplication.

        Only sequences which first appear in the first 100,000 sequences in each file are analysed. This should be enough to get a good impression for the duplication levels in the whole file. Each sequence is tracked to the end of the file to give a representative count of the overall duplication level.

        The duplication detection requires an exact sequence match over the whole length of the sequence. Any reads over 75bp in length are truncated to 50bp for this analysis.

        In a properly diverse library most sequences should fall into the far left of the plot in both the red and blue lines. A general level of enrichment, indicating broad oversequencing in the library will tend to flatten the lines, lowering the low end and generally raising other categories. More specific enrichments of subsets, or the presence of low complexity contaminants will tend to produce spikes towards the right of the plot.

        123456789>10>50>100>500>1k>5k>10k+0%20%40%60%80%100%
        FastQC: Sequence Duplication LevelsSequence Duplication Level% of Library
        Created with MultiQC

        Overrepresented sequences by sample
        3
        2
        0

        The total amount of overrepresented sequences found in each library.

        FastQC calculates and lists overrepresented sequences in FastQ files. It would not be possible to show this for all samples in a MultiQC report, so instead this plot shows the number of sequences categorized as overrepresented.

        Sometimes, a single sequence may account for a large number of reads in a dataset. To show this, the bars are split into two: the first shows the overrepresented reads that come from the single most common sequence. The second shows the total count from all remaining overrepresented sequences.

        From the FastQC Help:

        A normal high-throughput library will contain a diverse set of sequences, with no individual sequence making up a tiny fraction of the whole. Finding that a single sequence is very overrepresented in the set either means that it is highly biologically significant, or indicates that the library is contaminated, or not as diverse as you expected.

        FastQC lists all the sequences which make up more than 0.1% of the total. To conserve memory only sequences which appear in the first 100,000 sequences are tracked to the end of the file. It is therefore possible that a sequence which is overrepresented but doesn't appear at the start of the file for some reason could be missed by this module.

        5 samples had less than 1% of reads made up of overrepresented sequences

        Top overrepresented sequences

        Top overrepresented sequences across all samples. The table shows 20 most overrepresented sequences across all samples, ranked by the number of samples they occur in.

        Showing 3/3 rows and 3/3 columns.
        Overrepresented sequence
        Samples
        Occurrences
        % of all reads
        AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGCGGTTATCTCGTA
        1
        1284211
        0.0416%
        AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGCGGTTATCGCGTA
        1
        749391
        0.0243%
        AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGAACCGCGGTGTAGATC
        1
        955240
        0.0309%

        Adapter Content
        1
        1
        3

        The cumulative percentage count of the proportion of your library which has seen each of the adapter sequences at each position.

        Note that only samples with ≥ 0.1% adapter contamination are shown.

        There may be several lines per sample, as one is shown for each adapter detected in the file.

        From the FastQC Help:

        The plot shows a cumulative percentage count of the proportion of your library which has seen each of the adapter sequences at each position. Once a sequence has been seen in a read it is counted as being present right through to the end of the read so the percentages you see will only increase as the read length goes on.

        204060801001201400%20%40%60%80%100%
        FastQC: Adapter ContentPosition (bp)% of Sequences
        Created with MultiQC

        Status Checks

        Status for each FastQC section showing whether results seem entirely normal (green), slightly abnormal (orange) or very unusual (red).

        FastQC assigns a status for each section of the report. These give a quick evaluation of whether the results of the analysis seem entirely normal (green), slightly abnormal (orange) or very unusual (red).

        It is important to stress that although the analysis results appear to give a pass/fail result, these evaluations must be taken in the context of what you expect from your library. A 'normal' sample as far as FastQC is concerned is random and diverse. Some experiments may be expected to produce libraries which are biased in particular ways. You should treat the summary evaluations therefore as pointers to where you should concentrate your attention and understand why your library may not look random and diverse.

        Specific guidance on how to interpret the output of each module can be found in the relevant report section, or in the FastQC help.

        In this heatmap, we summarise all of these into a single heatmap for a quick overview. Note that not all FastQC sections have plots in MultiQC reports, but all status checks are shown in this heatmap.

        Basic StatisticsPer Base Sequence QualityPer Tile Sequence QualityPer Sequence Quality ScoresPer Base Sequence ContentPer Sequence GC ContentPer Base N ContentSequence Length DistributionSequence Duplication LevelsOverrepresented SequencesAdapter Contentin4276_5in4276_4in4276_3in4276_2in4276_1
        FastQC: Status Checks
        Created with MultiQC

        FastP (Read preprocessing)

        FastP (Read preprocessing) An ultra-fast all-in-one FASTQ preprocessor (QC, adapters, trimming, filtering, splitting...).DOI: 10.1093/bioinformatics/bty560.

        Filtered Reads

        Filtering statistics of sampled reads.

        00.2B0.4B0.6B0.8B1B1.2B1.4Bin4276_5in4276_4in4276_3in4276_2in4276_1
        Passed FilterLow QualityToo Many NToo ShortToo LongFastp: Filtered Reads# Reads
        Created with MultiQC

        Insert Sizes

        Insert size estimation of sampled reads.

        501001502002500%0.2%0.4%0.6%0.8%1%1.2%
        Fastp: Insert Size DistributionInsert sizeRead percent
        Created with MultiQC

        Sequence Quality

        Average sequencing quality over each base of all reads.

        2040608010012014005101520253035
        Fastp: Sequence QualityRead PositionR1 Before filtering: Sequence Quality
        Created with MultiQC

        GC Content

        Average GC content over each base of all reads.

        204060801001201400%20%40%60%80%100%
        Fastp: Read GC ContentRead PositionR1 Before filtering: Base Content Percent
        Created with MultiQC

        N content

        Average N content over each base of all reads.

        204060801001201400%20%40%60%80%100%
        Fastp: Read N ContentRead PositionR1 Before filtering: Base Content Percent
        Created with MultiQC

        GATK4 BQSR

        GATK is a toolkit offering a wide variety of tools with a primary focus on variant discovery and genotyping.DOI: 10.1101/201178; 10.1002/0471250953.bi1110s43; 10.1038/ng.806; 10.1101/gr.107524.110.

        Observed Quality Scores

        This plot shows the distribution of base quality scores in each sample before and after base quality score recalibration (BQSR). Applying BQSR should broaden the distribution of base quality scores.

        For more information see the Broad's description of BQSR.

        0102030405060708090020B40B60B80B100B120B140B160B
        GATK: Observed Quality Score CountsObserved Quality ScoreCount
        Created with MultiQC

        Reported Quality vs. Empirical Quality

        Plot shows the reported quality score vs the empirical quality score.

        010203005101520253035
        Reported vs. Empirical QualityReported quality scoreEmpirical quality score
        Created with MultiQC

        Vcftools

        Vcftools is a program for working with and reporting on VCF files.DOI: 10.1093/bioinformatics/btr330.

        TsTv by Count

        Plot of TSTV-BY-COUNT - the transition to transversion ratio as a function of alternative allele count from the output of vcftools TsTv-by-count.

        Transition is a purine-to-purine or pyrimidine-to-pyrimidine point mutations. Transversion is a purine-to-pyrimidine or pyrimidine-to-purine point mutation. Alternative allele count is the number of alternative alleles at the site. Note: only bi-allelic SNPs are used (multi-allelic sites and INDELs are skipped.) Refer to Vcftools's manual (https://vcftools.github.io/man_latest.html) on --TsTv-by-count

        00.20.40.60.8100.511.52
        VCFTools: TsTv by CountAlternative Allele CountTsTv Ratio
        Created with MultiQC

        TsTv by Qual

        Plot of TSTV-BY-QUAL - the transition to transversion ratio as a function of SNP quality from the output of vcftools TsTv-by-qual.

        Transition is a purine-to-purine or pyrimidine-to-pyrimidine point mutations. Transversion is a purine-to-pyrimidine or pyrimidine-to-purine point mutation. Quality here is the Phred-scaled quality score as given in the QUAL column of VCF. Note: only bi-allelic SNPs are used (multi-allelic sites and INDELs are skipped.) Refer to Vcftools's manual (https://vcftools.github.io/man_latest.html) on --TsTv-by-qual

        010203040506070800123456789
        VCFTools: TsTv by QualSNP Quality ThresholdTsTv Ratio
        Created with MultiQC

        Software Versions

        Software Versions lists versions of software tools extracted from file contents.

        GroupSoftwareVersion
        BCFTOOLS_STATSbcftools1.18
        BWAMEM1_MEMbwa0.7.17.post1188
        samtools1.19.2
        CRAM_TO_BAMsamtools1.19.2
        CRAM_TO_BAM_RECALsamtools1.19.2
        DEEPVARIANTdeepvariant1.5.0
        FASTPfastp0.23.4
        FASTQCfastqc0.12.1
        GATK4 MarkDuplicatesgatk44.5.0.0
        samtools1.19.2
        GATK4_APPLYBQSRgatk44.5.0.0
        GATK4_BASERECALIBRATORgatk44.5.0.0
        GATK4_GATHERBQSRREPORTSgatk44.5.0.0
        INDEX_CRAMsamtools1.19.2
        INDEX_MERGE_BAMsamtools1.19.2
        MERGE_BAMsamtools1.19.2
        MERGE_CRAMsamtools1.19.2
        MERGE_DEEPVARIANT_GVCFgatk44.5.0.0
        MERGE_DEEPVARIANT_VCFgatk44.5.0.0
        Mosdepthmosdepth0.3.6
        SAMTOOLS_STATSsamtools1.19.2
        SNPEFF_SNPEFFsnpeff5.1d
        TABIX_BGZIPTABIXtabix1.19.1
        VCFTOOLS_TSTV_COUNTvcftools0.1.16
        WorkflowNextflow23.10.1
        nf-core/sarek3.4.1

        nf-core/sarek Methods Description

        Suggested text and references to use when describing pipeline usage within the methods section of a publication.

        Methods

        Data was processed using nf-core/sarek v3.4.1 (doi: 10.12688/f1000research.16665.2, 10.1101/2023.07.19.549462, 10.5281/zenodo.3476425) of the nf-core collection of workflows (Ewels et al., 2020), utilising reproducible software environments from the Bioconda (Grüning et al., 2018) and Biocontainers (da Veiga Leprevost et al., 2017) projects.

        The pipeline was executed with Nextflow v23.10.1 (Di Tommaso et al., 2017) with the following command:

        nextflow run /home/hpatel/sarek/sarek_custom/sarek/main.nf --input /home/hpatel/in4276.csv --outdir 's3://zymo-filesystem/home/hpatel/in4276_logo_sarek_run/in4276/' -w /mnt/workdir/hpatel/ -profile slurm,apptainer --partition devel --genome null --igenomes_ignore --fasta 's3://zymo-filesystem/home/hpatel/reference_genomes/GIAB/GRCh38_GIABv3_no_alt_analysis_set_maskedGRC_decoys_MAP2K3_KMT2C_KCNJ18.fasta' --trim_fastq true --save_trimmed true --save_mapped true --save_output_as_bam true --dbsnp 's3://zymo-filesystem/home/hpatel/reference_genomes/GRCh38/Homo_sapiens/GATK/GRCh38/Annotation/GATKBundle/dbsnp_146.hg38.vcf.gz' --dbsnp_tbi 's3://zymo-filesystem/home/hpatel/reference_genomes/GRCh38/Homo_sapiens/GATK/GRCh38/Annotation/GATKBundle/dbsnp_146.hg38.vcf.gz.tbi' --known_indels 's3://zymo-filesystem/home/hpatel/reference_genomes/GRCh38/Homo_sapiens/GATK/GRCh38/Annotation/GATKBundle/Mills_and_1000G_gold_standard.indels.hg38.vcf.gz' --known_indels_tbi 's3://zymo-filesystem/home/hpatel/reference_genomes/GRCh38/Homo_sapiens/GATK/GRCh38/Annotation/GATKBundle/Mills_and_1000G_gold_standard.indels.hg38.vcf.gz.tbi' --snpeff_cache 's3://zymo-filesystem/home/hpatel/sarek/Annotation/snpeff_cache/' --snpeff_db 105 --snpeff_genome GRCh38 --tools deepvariant,snpeff --multiqc_config /home/hpatel/sarek/multiqc_custom_config.yml --custom_config_base /home/hpatel/sarek/ -resume

        References

        • Di Tommaso, P., Chatzou, M., Floden, E. W., Barja, P. P., Palumbo, E., & Notredame, C. (2017). Nextflow enables reproducible computational workflows. Nature Biotechnology, 35(4), 316-319. doi: 10.1038/nbt.3820
        • Ewels, P. A., Peltzer, A., Fillinger, S., Patel, H., Alneberg, J., Wilm, A., Garcia, M. U., Di Tommaso, P., & Nahnsen, S. (2020). The nf-core framework for community-curated bioinformatics pipelines. Nature Biotechnology, 38(3), 276-278. doi: 10.1038/s41587-020-0439-x
        • Grüning, B., Dale, R., Sjödin, A., Chapman, B. A., Rowe, J., Tomkins-Tinch, C. H., Valieris, R., Köster, J., & Bioconda Team. (2018). Bioconda: sustainable and comprehensive software distribution for the life sciences. Nature Methods, 15(7), 475–476. doi: 10.1038/s41592-018-0046-7
        • da Veiga Leprevost, F., Grüning, B. A., Alves Aflitos, S., Röst, H. L., Uszkoreit, J., Barsnes, H., Vaudel, M., Moreno, P., Gatto, L., Weber, J., Bai, M., Jimenez, R. C., Sachsenberg, T., Pfeuffer, J., Vera Alvarez, R., Griss, J., Nesvizhskii, A. I., & Perez-Riverol, Y. (2017). BioContainers: an open-source and community-driven framework for software standardization. Bioinformatics (Oxford, England), 33(16), 2580–2582. doi: 10.1093/bioinformatics/btx192
        Notes:
        • The command above does not include parameters contained in any configs or profiles that may have been used. Ensure the config file is also uploaded with your publication!
        • You should also cite all software used within this run. Check the "Software Versions" of this report to get version information.

        nf-core/sarek Workflow Summary

        - this information is collected when the pipeline is started.

        Core Nextflow options

        runName
        small_lavoisier
        containerEngine
        apptainer
        launchDir
        /mnt/home/hpatel/in4276
        workDir
        /mnt/workdir/hpatel
        projectDir
        /home/hpatel/sarek/sarek_custom/sarek
        userName
        hpatel
        profile
        slurm,apptainer
        configFiles
        N/A

        Input/output options

        input
        /home/hpatel/in4276.csv
        outdir
        s3://zymo-filesystem/home/hpatel/in4276_logo_sarek_run/in4276/

        Main options

        tools
        deepvariant,snpeff

        FASTQ Preprocessing

        trim_fastq
        true
        save_trimmed
        true

        Preprocessing

        save_mapped
        true
        save_output_as_bam
        true

        Reference genome options

        genome
        null
        dbsnp
        s3://zymo-filesystem/home/hpatel/reference_genomes/GRCh38/Homo_sapiens/GATK/GRCh38/Annotation/GATKBundle/dbsnp_146.hg38.vcf.gz
        dbsnp_tbi
        s3://zymo-filesystem/home/hpatel/reference_genomes/GRCh38/Homo_sapiens/GATK/GRCh38/Annotation/GATKBundle/dbsnp_146.hg38.vcf.gz.tbi
        fasta
        s3://zymo-filesystem/home/hpatel/reference_genomes/GIAB/GRCh38_GIABv3_no_alt_analysis_set_maskedGRC_decoys_MAP2K3_KMT2C_KCNJ18.fasta
        known_indels
        s3://zymo-filesystem/home/hpatel/reference_genomes/GRCh38/Homo_sapiens/GATK/GRCh38/Annotation/GATKBundle/Mills_and_1000G_gold_standard.indels.hg38.vcf.gz
        known_indels_tbi
        s3://zymo-filesystem/home/hpatel/reference_genomes/GRCh38/Homo_sapiens/GATK/GRCh38/Annotation/GATKBundle/Mills_and_1000G_gold_standard.indels.hg38.vcf.gz.tbi
        snpeff_db
        105
        snpeff_genome
        GRCh38
        igenomes_ignore
        true
        snpeff_cache
        s3://zymo-filesystem/home/hpatel/sarek/Annotation/snpeff_cache/

        Institutional config options

        custom_config_base
        /home/hpatel/sarek/

        Generic options

        multiqc_config
        /home/hpatel/sarek/multiqc_custom_config.yml
        validationLenientMode
        true