FastQCFastQC Report
Tue 23 Feb 2021
in2197_12_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamein2197_12_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10000000
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACACTAAGATCTCGTA2033862.03386TruSeq Adapter, Index 5 (97% over 38bp)
TCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACACTAAGATCTCG767880.76788TruSeq Adapter, Index 5 (97% over 38bp)
CTGGGCTGTTTCCCTCTTGACAACGGACCTTATCACCCGCTGTCTGACTG234500.2345No Hit
CTGAGCCAACCTCCTGGCTGTCTATGCGACTCCACATCCTTTCCCACTTA225900.22590000000000002No Hit
CCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACACTAAGATCTCG136560.13656000000000001TruSeq Adapter, Index 5 (97% over 38bp)
CTCTCGTATTTAGCCTTGCCGGATGGTCCCGGCTGATTCAGACAGGATTT123470.12347No Hit
CTCAGTCCGGCTACCGATCGCGGTCTTGGTGAGCCGTTACCTCACCAACT112540.11253999999999999No Hit
CTCACTAGAAGCTTTTCTCGACAGCATGGCTCACTTGAATTTGACTTTAC111450.11145000000000001No Hit
CGTCGCCTTGGTGGGCCTTTACCCTCACCAACTAGCTAATCAGACGCAAG109020.10901999999999999No Hit
CTCTCGCTCACCTTAGGATTCTCTCCTCACCCATGTGTGTCCATTTTCGG107120.10712No Hit
CTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACACTAAGATCTCG106200.1062TruSeq Adapter, Index 5 (97% over 38bp)
CTTTAAATGAATGGCTGCTTCCAAGCCAACATCCTAGCTGTCTTAGCAAT105670.10567No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)