FastQCFastQC Report
Tue 23 Feb 2021
in2197_14_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamein2197_14_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10000000
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTCGTGTATCTCGTA2087742.08774TruSeq Adapter, Index 8 (97% over 38bp)
TCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTCGTGTATCTCG661350.66135TruSeq Adapter, Index 8 (97% over 38bp)
CTGAGCCAACCTCCTGGCTGTCTATGCGACTCCACATCCTTTCCCACTTA213750.21375No Hit
CTGGGCTGTTTCCCTCTTGACAACGGACCTTATCACCCGCTGTCTGACTG185070.18507No Hit
CTCTCGTATTTAGCCTTGCCGGATGGTCCCGGCTGATTCAGACAGGATTT134650.13465No Hit
CGTCGCCTTGGTGGGCCTTTACCCTCACCAACTAGCTAATCAGACGCAAG121770.12176999999999999No Hit
CTCTCGTATTTAGCCTTGCCGGATGGTCCCGGCCGATTCAGACAGGATTT106520.10652No Hit
CTCAGTCCGGCTACCGATCGCGGTCTTGGTGAGCCGTTACCTCACCAACT105380.10537999999999999No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)