FastQCFastQC Report
Tue 23 Feb 2021
in2197_15_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamein2197_15_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10000000
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCCTGTTATCTCGTA2447402.4474TruSeq Adapter, Index 18 (97% over 37bp)
TCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCCTGTTATCTCG909700.9097TruSeq Adapter, Index 18 (97% over 37bp)
CTGAGCCAACCTCCTGGCTGTCTATGCGACTCCACATCCTTTCCCACTTA228900.2289No Hit
CTGGGCTGTTTCCCTCTTGACAACGGACCTTATCACCCGCTGTCTGACTG169600.1696No Hit
CTCTCGTATTTAGCCTTGCCGGATGGTCCCGGCTGATTCAGACAGGATTT133670.13366999999999998No Hit
CTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCCTGTTATCTCG120370.12037TruSeq Adapter, Index 18 (97% over 37bp)
CGTCGCCTTGGTGGGCCTTTACCCTCACCAACTAGCTAATCAGACGCAAG108380.10838No Hit
CTCAGTCCGGCTACCGATCGCGGTCTTGGTGAGCCGTTACCTCACCAACT100130.10013000000000001No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)