FastQCFastQC Report
Tue 23 Feb 2021
in2197_16_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamein2197_16_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10000000
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAGGTCAATCTCGTA3289343.28934TruSeq Adapter, Index 3 (97% over 38bp)
TCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAGGTCAATCTCG1204061.2040600000000001TruSeq Adapter, Index 3 (97% over 38bp)
CTGAGCCAACCTCCTGGCTGTCTATGCGACTCCACATCCTTTCCCACTTA188100.18810000000000002No Hit
CTGGGCTGTTTCCCTCTTGACAACGGACCTTATCACCCGCTGTCTGACTG182590.18259No Hit
CTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAGGTCAATCTCG160060.16006TruSeq Adapter, Index 3 (97% over 38bp)
CCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAGGTCAATCTCG147240.14724TruSeq Adapter, Index 3 (97% over 38bp)
CTTTAAATGAATGGCTGCTTCCAAGCCAACATCCTAGCTGTCTTAGCAAT117740.11774000000000001No Hit
CTCTCGTATTTAGCCTTGCCGGATGGTCCCGGCTGATTCAGACAGGATTT112750.11275No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)