FastQCFastQC Report
Tue 23 Feb 2021
in2197_17_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamein2197_17_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10000000
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCATTCGAATCTCGTA2617602.6176TruSeq Adapter, Index 23 (97% over 39bp)
TCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCATTCGAATCTCG967480.9674800000000001TruSeq Adapter, Index 23 (97% over 39bp)
CTGAGCCAACCTCCTGGCTGTCTATGCGACTCCACATCCTTTCCCACTTA267590.26759No Hit
CTGGGCTGTTTCCCTCTTGACAACGGACCTTATCACCCGCTGTCTGACTG202910.20290999999999998No Hit
CTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCATTCGAATCTCG132610.13261TruSeq Adapter, Index 23 (97% over 39bp)
CTCTCGTATTTAGCCTTGCCGGATGGTCCCGGCTGATTCAGACAGGATTT125770.12577No Hit
CCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCATTCGAATCTCG125470.12547TruSeq Adapter, Index 23 (97% over 39bp)
CTCACTAGAAGCTTTTCTCGACAGCATGGCTCACTTGAATTTGACTTTAC114270.11427No Hit
CGTCGCCTTGGTGGGCCTTTACCCTCACCAACTAGCTAATCAGACGCAAG107320.10732000000000001No Hit
CTCAGTCCGGCTACCGATCGCGGTCTTGGTGAGCCGTTACCTCACCAACT102070.10207000000000001No Hit
CTTTAAATGAATGGCTGCTTCCAAGCCAACATCCTAGCTGTCTTAGCAAT101350.10135000000000001No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)