FastQCFastQC Report
Tue 23 Feb 2021
in2197_1_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamein2197_1_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10000000
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGAACTGATCTCGTA2595252.59525TruSeq Adapter, Index 7 (97% over 37bp)
TCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGAACTGATCTCG929120.92912TruSeq Adapter, Index 7 (97% over 37bp)
CTGAGCCAACCTCCTGGCTGTCTATGCGACTCCACATCCTTTCCCACTTA175360.17536No Hit
CTGGGCTGTTTCCCTCTTGACAACGGACCTTATCACCCGCTGTCTGACTG165500.1655No Hit
CTCTCGTATTTAGCCTTGCCGGATGGTCCCGGCTGATTCAGACAGGATTT123850.12385No Hit
CTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGAACTGATCTCG121910.12191TruSeq Adapter, Index 7 (97% over 37bp)
CTTTAAATGAATGGCTGCTTCCAAGCCAACATCCTAGCTGTCTTAGCAAT121190.12118999999999999No Hit
CCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGAACTGATCTCG117490.11749TruSeq Adapter, Index 7 (97% over 37bp)
CTCTTTAAATGAATGGCTGCTTCCAAGCCAACATCCTAGCTGTCTTAGCA106570.10657No Hit
CTCTCGCTCACCTTAGGATTCTCTCCTCACCCATGTGTGTCCATTTTCGG106300.10629999999999999No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)