FastQCFastQC Report
Tue 23 Feb 2021
in2197_20_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamein2197_20_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10000000
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTCACGAATCTCG658330.6583300000000001TruSeq Adapter, Index 9 (97% over 38bp)
TCCCCGTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGTG432320.43232000000000004No Hit
CCCCCGTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGTG365180.36518No Hit
CCCCGGACATTTTCGGCGCAGGACCTCTCGACTAGTGAGCTATTACGCAC264910.26491000000000003No Hit
CCCCGTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGTGG225660.22566No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTCACGAATCTCGTA225090.22509TruSeq Adapter, Index 9 (97% over 38bp)
GCCCCGGACATTTTCGGCGCAGGACCTCTCGACTAGTGAGCTATTACGCA194190.19419No Hit
CCCCCGTCAATTCCTTTGAGTTTCACCGTTGCCGGCGTACTCCCCAGGTG193910.19391No Hit
CCCCGGACATTTTCGGCGCAGGACCTCTCGACCAGTGAGCTATTACGCAC176250.17625No Hit
TCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTCACGAATCTCG175980.17598TruSeq Adapter, Index 9 (97% over 38bp)
CCCCCGTCAATTCCTTTGAGTTTCAACCTTGCGGTCGTACTCCCCAGGTG160610.16061No Hit
CTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTCACGAATCTCG152330.15233TruSeq Adapter, Index 9 (97% over 38bp)
CTCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTCACGAATC145470.14547TruSeq Adapter, Index 9 (97% over 38bp)
CCCCGGACATTTTCGGCGCAGGAACTCTCGACTAGTGAGCTGTTACGCAC133940.13394No Hit
TCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTCACGAATC130340.13033999999999998TruSeq Adapter, Index 9 (97% over 38bp)
CCGCCTTCGCCACTGGTGTTCCTCCTAATATCTACGCATTTCACCGCTAC124720.12472No Hit
CCCCGGACATTTTCGGCGCAGGGACTCTCGACTAGTGAGCTGTTACGCAC120990.12099000000000001No Hit
ACCCCGTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGTG119090.11909No Hit
CCCCCGTTAAATTCTCCGCGCAAAGACGCTCGACCAGTGAGCTATTACGC117070.11707No Hit
CCCACGCCGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTC107530.10753No Hit
CCCGGACATTTTCGGCGCAGGACCTCTCGACTAGTGAGCTATTACGCACT105790.10579000000000001No Hit
GCCCCGGACATTTTCGGCGCAGGACCTCTCGACCAGTGAGCTATTACGCA103910.10391No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)