FastQCFastQC Report
Tue 23 Feb 2021
in2197_20_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamein2197_20_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10000000
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCATAATACGTGTAGA665890.66589Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGAC319050.31905No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCATAATACGTGTAGATC228360.22836Illumina Single End PCR Primer 1 (96% over 33bp)
GGGATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACC216670.21667No Hit
GGGGATAACAGGCTTATCACTCCCAAGAGTTCACATCGACGGAGTGGTTT206940.20693999999999999No Hit
GAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCATAATACGTGTAGA177310.17731Illumina Single End PCR Primer 1 (96% over 33bp)
AGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCATAATACGTGTAGA154370.15437Illumina Single End PCR Primer 1 (96% over 33bp)
GGGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCATAATACGTGT143580.14358Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCATAATACGTGT129140.12914Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGATAACGGTCCTAAGGTAGCGAAATTCCTTGTCAGGTAAGTTCTGAC114690.11469No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)