FastQCFastQC Report
Tue 23 Feb 2021
in2197_21_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamein2197_21_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5305703
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGCTTCCATCTCG282990.5333694705489546TruSeq Adapter, Index 7 (97% over 36bp)
TCCCCGTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGTG240690.4536439374763344No Hit
CCCCCGTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGTG213830.40301916635740825No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGCTTCCATCTCGTA129890.24481204469982581TruSeq Adapter, Index 7 (97% over 36bp)
CCCCGTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGTGG124790.23519974638610566No Hit
CCCCGGACATTTTCGGCGCAGGACCTCTCGACTAGTGAGCTATTACGCAC121080.22820727055396806No Hit
GCCCCGGACATTTTCGGCGCAGGACCTCTCGACTAGTGAGCTATTACGCA102920.19397994949962333No Hit
CCCCCGTCAATTCCTTTGAGTTTCACCGTTGCCGGCGTACTCCCCAGGTG92010.17341717016576313No Hit
TCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGCTTCCATCTCG91600.17264441677191505TruSeq Adapter, Index 7 (97% over 36bp)
CCCCCGTCAATTCCTTTGAGTTTCAACCTTGCGGTCGTACTCCCCAGGTG88600.1669901236461973No Hit
CTCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGCTTCCATC87390.16470955875215781TruSeq Adapter, Index 7 (97% over 36bp)
CCCCGGACATTTTCGGCGCAGGACCTCTCGACCAGTGAGCTATTACGCAC82560.15560614681975224No Hit
CCGCCTTCGCCACTGGTGTTCCTCCTAATATCTACGCATTTCACCGCTAC79400.14965029139399624No Hit
TCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGCTTCCATC75550.14239394854932513TruSeq Adapter, Index 7 (97% over 36bp)
CTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGCTTCCATCTCG72610.13685274128612174TruSeq Adapter, Index 7 (97% over 36bp)
ACCCCGTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGTG69390.13078379999785136No Hit
CCCCCGTTAAATTCTCCGCGCAAAGACGCTCGACCAGTGAGCTATTACGC66920.12612843199101043No Hit
CCCCGGACATTTTCGGCGCAGGGACTCTCGACTAGTGAGCTGTTACGCAC61180.11530988447713715No Hit
CCCCGGACATTTTCGGCGCAGGAACTCTCGACTAGTGAGCTGTTACGCAC58630.11050373532027707No Hit
CCCACGCCGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTC56430.10635725369475073No Hit
CCCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAATGT56190.1059049102446933No Hit
CCCCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAATG53450.10074065585653777No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)