FastQCFastQC Report
Tue 23 Feb 2021
in2197_21_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamein2197_21_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5305703
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGATCTATCGTGTAGA286660.5402865558060826Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGAC182030.3430836592248002No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGATCTATCGTGTAGATC132010.24880774517533305Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGATAACAGGCTTATCACTCCCAAGAGTTCACATCGACGGAGTGGTTT120650.22739682187261517No Hit
GGGATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACC107990.2035357048820863No Hit
GAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGATCTATCGTGTAGA93230.17571658270355503Illumina Single End PCR Primer 1 (96% over 33bp)
GGGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGATCTATCGTGT86280.16261747029564227Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGATCTATCGTGT74740.14086728940538135Illumina Single End PCR Primer 1 (96% over 33bp)
AGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGATCTATCGTGTAGA73740.1389825250301421Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGATAACGGTCCTAAGGTAGCGAAATTCCTTGTCAGGTAAGTTCTGAC67730.1276550911349542No Hit
GGGGACCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGA54480.1026819631630342No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)