FastQCFastQC Report
Tue 23 Feb 2021
in2197_22_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamein2197_22_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10000000
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCATCCTTATCTCG653360.6533599999999999TruSeq Adapter, Index 3 (97% over 36bp)
TCCCCGTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGTG419420.41942No Hit
CCCCCGTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGTG393870.39387No Hit
CCCCGGACATTTTCGGCGCAGGACCTCTCGACTAGTGAGCTATTACGCAC235420.23542No Hit
CCCCGTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGTGG218080.21808No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCATCCTTATCTCGTA197510.19751TruSeq Adapter, Index 3 (97% over 36bp)
TCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCATCCTTATCTCG196660.19666000000000003TruSeq Adapter, Index 3 (97% over 36bp)
CTCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCATCCTTATC191010.19101TruSeq Adapter, Index 3 (97% over 36bp)
CCCCGGACATTTTCGGCGCAGGACCTCTCGACCAGTGAGCTATTACGCAC186370.18637No Hit
TCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCATCCTTATC171770.17177TruSeq Adapter, Index 3 (97% over 36bp)
GCCCCGGACATTTTCGGCGCAGGACCTCTCGACTAGTGAGCTATTACGCA162600.1626No Hit
CTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCATCCTTATCTCG162350.16235TruSeq Adapter, Index 3 (97% over 36bp)
CCCCCGTCAATTCCTTTGAGTTTCAACCTTGCGGTCGTACTCCCCAGGTG159540.15954000000000002No Hit
CCCACGCCGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTC158010.15801No Hit
CCGCCTTCGCCACTGGTGTTCCTCCTAATATCTACGCATTTCACCGCTAC150780.15078No Hit
ACCCCGTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGTG125780.12578No Hit
CCCCGGACATTTTCGGCGCAGGGACTCTCGACTAGTGAGCTGTTACGCAC106180.10618No Hit
CCCCCGTCAATTCCTTTGAGTTTCACCGTTGCCGGCGTACTCCCCAGGTG104860.10486No Hit
GCCCCGGACATTTTCGGCGCAGGACCTCTCGACCAGTGAGCTATTACGCA104810.10481No Hit
CCCGGACATTTTCGGCGCAGGACCTCTCGACTAGTGAGCTATTACGCACT103150.10315No Hit
TCGCCACTGGTGTTCCTCCTAATATCTACGCATTTCACCGCTACACTAGG101270.10127No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)