FastQCFastQC Report
Tue 23 Feb 2021
in2197_22_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamein2197_22_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10000000
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGCTCGCTGTGTAGA661990.66199Illumina Single End PCR Primer 1 (97% over 38bp)
GGGGATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGAC342170.34217000000000003No Hit
GGGGATAACAGGCTTATCACTCCCAAGAGTTCACATCGACGGAGTGGTTT267150.26715No Hit
GGGATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACC224990.22499No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGCTCGCTGTGTAGATC200880.20087999999999998Illumina Single End PCR Primer 1 (97% over 38bp)
GAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGCTCGCTGTGTAGA200180.20018000000000002Illumina Single End PCR Primer 1 (97% over 38bp)
GGGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGCTCGCTGTGT189200.1892Illumina Single End PCR Primer 1 (97% over 38bp)
GGGGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGCTCGCTGTGT170400.1704Illumina Single End PCR Primer 1 (97% over 38bp)
AGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGCTCGCTGTGTAGA164040.16404Illumina Single End PCR Primer 1 (97% over 38bp)
GGGGATAACGGTCCTAAGGTAGCGAAATTCCTTGTCAGGTAAGTTCTGAC143270.14327No Hit
GGGATAACAGGCTTATCACTCCCAAGAGTTCACATCGACGGAGTGGTTTG119920.11992000000000001No Hit
GGGGACCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGA113000.11299999999999999No Hit
GGGGTAACAGGCTTATCACTCCCAAGAGTTCACATCGACGGAGTGGTTTG110570.11057No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)