FastQCFastQC Report
Tue 23 Feb 2021
in2197_23_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamein2197_23_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10000000
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCCGTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGTG487100.48710000000000003No Hit
CCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGTTATAATCTCG474980.47498TruSeq Adapter, Index 14 (97% over 37bp)
CCCCCGTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGTG390240.39024No Hit
CCCCGTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGTGG229220.22921999999999998No Hit
CCCCGGACATTTTCGGCGCAGGACCTCTCGACTAGTGAGCTATTACGCAC213470.21347No Hit
TCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGTTATAATCTCG194430.19443TruSeq Adapter, Index 14 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGTTATAATCTCGTA181820.18182TruSeq Adapter, Index 14 (97% over 37bp)
GCCCCGGACATTTTCGGCGCAGGACCTCTCGACTAGTGAGCTATTACGCA180050.18005No Hit
CTCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGTTATAATC169970.16996999999999998TruSeq Adapter, Index 14 (97% over 37bp)
CCGCCTTCGCCACTGGTGTTCCTCCTAATATCTACGCATTTCACCGCTAC163170.16317No Hit
CCCCCGTCAATTCCTTTGAGTTTCAACCTTGCGGTCGTACTCCCCAGGTG157140.15714No Hit
CCCCGGACATTTTCGGCGCAGGACCTCTCGACCAGTGAGCTATTACGCAC153200.1532No Hit
TCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGTTATAATC150380.15038TruSeq Adapter, Index 14 (97% over 37bp)
CCCCCGTCAATTCCTTTGAGTTTCACCGTTGCCGGCGTACTCCCCAGGTG144240.14423999999999998No Hit
CCCACGCCGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTC136690.13669No Hit
CTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGTTATAATCTCG131720.13172TruSeq Adapter, Index 14 (97% over 37bp)
CCCCCGTTAAATTCTCCGCGCAAAGACGCTCGACCAGTGAGCTATTACGC113890.11388999999999999No Hit
TCGCCACTGGTGTTCCTCCTAATATCTACGCATTTCACCGCTACACTAGG109970.10996999999999998No Hit
CCCGTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGTGGA109370.10937No Hit
CCCCGGACATTTTCGGCGCAGGAACTCTCGACTAGTGAGCTGTTACGCAC109060.10905999999999999No Hit
CCCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAATGT107780.10778No Hit
CCCCGGACATTTTCGGCGCAGGGACTCTCGACTAGTGAGCTGTTACGCAC105520.10552No Hit
CCCCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAATG103980.10398No Hit
ACCCCGTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGTG102310.10231000000000001No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)