FastQCFastQC Report
Tue 23 Feb 2021
in2197_23_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamein2197_23_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10000000
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCGGAACTGGTGTAGA481030.48103Illumina Single End PCR Primer 1 (97% over 35bp)
GGGGATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGAC379830.37983No Hit
GGGGATAACAGGCTTATCACTCCCAAGAGTTCACATCGACGGAGTGGTTT297900.2979No Hit
GGGATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACC233860.23386No Hit
GAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCGGAACTGGTGTAGA196610.19661Illumina Single End PCR Primer 1 (97% over 35bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCGGAACTGGTGTAGATC185500.1855Illumina Single End PCR Primer 1 (97% over 35bp)
GGGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCGGAACTGGTGT167910.16791Illumina Single End PCR Primer 1 (97% over 35bp)
GGGGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCGGAACTGGTGT149140.14914Illumina Single End PCR Primer 1 (97% over 35bp)
AGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCGGAACTGGTGTAGA132830.13283Illumina Single End PCR Primer 1 (97% over 35bp)
GGGGATAACGGTCCTAAGGTAGCGAAATTCCTTGTCAGGTAAGTTCTGAC127630.12763No Hit
GGGATAACAGGCTTATCACTCCCAAGAGTTCACATCGACGGAGTGGTTTG124680.12468No Hit
GGGGTAACAGGCTTATCACTCCCAAGAGTTCACATCGACGGAGTGGTTTG117410.11741No Hit
GGGGACCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGA109190.10919000000000001No Hit
GGGGGATAACAGGCTTATCACTCCCAAGAGTTCACATCGACGGAGTGGTT106650.10665No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)