FastQCFastQC Report
Tue 23 Feb 2021
in2197_24_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamein2197_24_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10000000
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAACCGCGATCTCG446970.44697TruSeq Adapter, Index 9 (97% over 37bp)
TCCCCGTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGTG446370.44637No Hit
CCCCCGTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGTG385360.38536No Hit
CCCCGTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGTGG225570.22557No Hit
CCCCGGACATTTTCGGCGCAGGACCTCTCGACTAGTGAGCTATTACGCAC218740.21874No Hit
CCCACGCCGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTC177790.17779No Hit
CCCCGGACATTTTCGGCGCAGGACCTCTCGACCAGTGAGCTATTACGCAC173540.17354No Hit
TCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAACCGCGATCTCG172030.17203TruSeq Adapter, Index 9 (97% over 37bp)
CCGCCTTCGCCACTGGTGTTCCTCCTAATATCTACGCATTTCACCGCTAC170140.17014No Hit
GCCCCGGACATTTTCGGCGCAGGACCTCTCGACTAGTGAGCTATTACGCA165210.16521000000000002No Hit
CCCCCGTCAATTCCTTTGAGTTTCAACCTTGCGGTCGTACTCCCCAGGTG149890.14989No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAACCGCGATCTCGTA143350.14335TruSeq Adapter, Index 9 (97% over 37bp)
CTCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAACCGCGATC134670.13466999999999998TruSeq Adapter, Index 9 (97% over 37bp)
CCCCCGTCAATTCCTTTGAGTTTCACCGTTGCCGGCGTACTCCCCAGGTG120190.12019No Hit
TCCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAACCGCGATC116820.11682000000000001TruSeq Adapter, Index 9 (97% over 37bp)
CTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAACCGCGATCTCG113480.11348TruSeq Adapter, Index 9 (97% over 37bp)
TCGCCACTGGTGTTCCTCCTAATATCTACGCATTTCACCGCTACACTAGG112120.11212No Hit
CCCCCGTTAAATTCTCCGCGCAAAGACGCTCGACCAGTGAGCTATTACGC107490.10748999999999999No Hit
CCCCGGACATTTTCGGCGCAGGGACTCTCGACTAGTGAGCTGTTACGCAC107080.10708No Hit
CCCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAATGT105260.10526No Hit
CCCCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAATG103950.10395No Hit
ACCCCGTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGTG101300.1013No Hit
CCCCGGACATTTTCGGCGCAGGAACTCTCGACTAGTGAGCTGTTACGCAC100520.10052No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)