FastQCFastQC Report
Tue 23 Feb 2021
in2197_24_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamein2197_24_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10000000
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGGTCAGTGTAGA458840.45884Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGAC399880.39988No Hit
GGGGATAACAGGCTTATCACTCCCAAGAGTTCACATCGACGGAGTGGTTT323800.3238No Hit
GGGATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACC265610.26561No Hit
GAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGGTCAGTGTAGA176420.17642Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGGTCAGTGTAGATC147620.14762Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGATAACGGTCCTAAGGTAGCGAAATTCCTTGTCAGGTAAGTTCTGAC143120.14312No Hit
GGGATAACAGGCTTATCACTCCCAAGAGTTCACATCGACGGAGTGGTTTG140410.14040999999999998No Hit
GGGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGGTCAGTGT134170.13416999999999998Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGTAACAGGCTTATCACTCCCAAGAGTTCACATCGACGGAGTGGTTTG122310.12231No Hit
GGGGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGGTCAGTGT116420.11642Illumina Single End PCR Primer 1 (96% over 33bp)
AGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGGTCAGTGTAGA116030.11603Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGATAACAGGCTTATCACTCCCAAGAGTTCACATCGACGGAGTGGTT110530.11053No Hit
GGGAGATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGA103660.10365999999999999No Hit
GGGGACCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGA100630.10063No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)