FastQCFastQC Report
Tue 23 Feb 2021
in2197_25_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamein2197_25_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10000000
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCGAGACATCTCGTA450380.45037999999999995TruSeq Adapter, Index 11 (97% over 36bp)
CTGAGCCAACCTCCTGGCTGTCTATGCGACTCCACATCCTTTCCCACTTA388450.38845No Hit
TCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCGAGACATCTCG160880.16088TruSeq Adapter, Index 11 (97% over 36bp)
CTGGGCTGTTTCCCTCTTGACAACGGACCTTATCACCCGCTGTCTGACTG160670.16067No Hit
CGTCGCCTTGGTGGGCCTTTACCCTCACCAACTAGCTAATCAGACGCAAG124220.12422No Hit
CTCAGGCCGGCTACCCGTCGTAGGCTCGGTGGGCCGTTACCCCGCCGACT120060.12006No Hit
CTCTCGTATTTAGCCTTGCCGGATGGTCCCGGCTGATTCAGACAGGATTT118200.11820000000000001No Hit
CTCAGTCCGGCTACCGATCGCGGTCTTGGTGAGCCGTTACCTCACCAACT118170.11817No Hit
CTCTCGCTCACCTTAGGATTCTCTCCTCACCCATGTGTGTCCATTTTCGG112440.11244No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)