FastQCFastQC Report
Tue 23 Feb 2021
in2197_26_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamein2197_26_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10000000
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATAGAGTATCTCGTA500300.5003TruSeq Adapter, Index 7 (97% over 37bp)
TCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATAGAGTATCTCG181250.18125000000000002TruSeq Adapter, Index 7 (97% over 37bp)
CTGGGCTGTTTCCCTCTTGACAACGGACCTTATCACCCGCTGTCTGACTG170270.17027No Hit
CTGAGCCAACCTCCTGGCTGTCTATGCGACTCCACATCCTTTCCCACTTA164590.16459000000000001No Hit
CTCAGTCCGGCTACCGATCGCGGTCTTGGTGAGCCGTTACCTCACCAACT144920.14492000000000002No Hit
CGTCGCCTTGGTGGGCCTTTACCCTCACCAACTAGCTAATCAGACGCAAG138650.13865No Hit
CTCTCGCTCACCTTAGGATTCTCTCCTCACCCATGTGTGTCCATTTTCGG129100.1291No Hit
CTCTCGTATTTAGCCTTGCCGGATGGTCCCGGCTGATTCAGACAGGATTT120860.12086No Hit
CTTTAAATGAATGGCTGCTTCCAAGCCAACATCCTAGCTGTCTTAGCAAT102410.10241No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)