FastQCFastQC Report
Tue 23 Feb 2021
in2197_28_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamein2197_28_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10000000
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAATATATCTCGTA523720.52372TruSeq Adapter, Index 19 (97% over 38bp)
CTGAGCCAACCTCCTGGCTGTCTATGCGACTCCACATCCTTTCCCACTTA198920.19891999999999999No Hit
TCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAATATATCTCG191230.19123TruSeq Adapter, Index 19 (97% over 38bp)
CTGGGCTGTTTCCCTCTTGACAACGGACCTTATCACCCGCTGTCTGACTG157660.15766No Hit
CGTCGCCTTGGTGGGCCTTTACCCTCACCAACTAGCTAATCAGACGCAAG143690.14369No Hit
CTCTCGTATTTAGCCTTGCCGGATGGTCCCGGCTGATTCAGACAGGATTT141220.14121999999999998No Hit
CTCAGTCCGGCTACCGATCGCGGTCTTGGTGAGCCGTTACCTCACCAACT136940.13694No Hit
CTCTCGCTCACCTTAGGATTCTCTCCTCACCCATGTGTGTCCATTTTCGG126430.12643000000000001No Hit
CTCTCGTATTTAGCCTTGCCGGATGGTCCCGGCCGATTCAGACAGGATTT111520.11152No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)