FastQCFastQC Report
Tue 23 Feb 2021
in2197_2_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamein2197_2_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10000000
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGCCTAGATCTCGTA4072604.0725999999999996TruSeq Adapter, Index 10 (97% over 37bp)
TCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGCCTAGATCTCG1417041.41704TruSeq Adapter, Index 10 (97% over 37bp)
CTGAGCCAACCTCCTGGCTGTCTATGCGACTCCACATCCTTTCCCACTTA216290.21629No Hit
CTGGGCTGTTTCCCTCTTGACAACGGACCTTATCACCCGCTGTCTGACTG195530.19553No Hit
CTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGCCTAGATCTCG186650.18665TruSeq Adapter, Index 10 (97% over 37bp)
CCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGCCTAGATCTCG161530.16153TruSeq Adapter, Index 10 (97% over 37bp)
CTCTCGTATTTAGCCTTGCCGGATGGTCCCGGCTGATTCAGACAGGATTT106640.10663999999999998No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)