FastQCFastQC Report
Tue 23 Feb 2021
in2197_4_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamein2197_4_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10000000
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCTCGCTATCTCGTA1762331.7623300000000002TruSeq Adapter, Index 14 (97% over 38bp)
TCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCTCGCTATCTCG672560.67256TruSeq Adapter, Index 14 (97% over 38bp)
CTGAGCCAACCTCCTGGCTGTCTATGCGACTCCACATCCTTTCCCACTTA214000.214No Hit
CTGGGCTGTTTCCCTCTTGACAACGGACCTTATCACCCGCTGTCTGACTG185300.1853No Hit
CTCTCGTATTTAGCCTTGCCGGATGGTCCCGGCTGATTCAGACAGGATTT135730.13573000000000002No Hit
CTTTAAATGAATGGCTGCTTCCAAGCCAACATCCTAGCTGTCTTAGCAAT110710.11071No Hit
CTCTCGTATTTAGCCTTGCCGGATGGTCCCGGCCGATTCAGACAGGATTT108140.10814No Hit
CTCACTAGAAGCTTTTCTCGACAGCATGGCTCACTTGAATTTGACTTTAC105670.10567No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)