FastQCFastQC Report
Tue 23 Feb 2021
in2197_5_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamein2197_5_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10000000
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTACTCCATCTCGTA635610.63561TruSeq Adapter, Index 14 (97% over 38bp)
CTGAGCCAACCTCCTGGCTGTCTATGCGACTCCACATCCTTTCCCACTTA247550.24755No Hit
TCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTACTCCATCTCG244900.24489999999999998TruSeq Adapter, Index 14 (97% over 38bp)
CTGGGCTGTTTCCCTCTTGACAACGGACCTTATCACCCGCTGTCTGACTG165930.16593No Hit
CTCTCGCTCACCTTAGGATTCTCTCCTCACCCATGTGTGTCCATTTTCGG131850.13185No Hit
CTCTCGTATTTAGCCTTGCCGGATGGTCCCGGCTGATTCAGACAGGATTT128930.12893No Hit
CGTCGCCTTGGTGGGCCTTTACCCTCACCAACTAGCTAATCAGACGCAAG120290.12029000000000001No Hit
CTCAGTCCGGCTACCGATCGCGGTCTTGGTGAGCCGTTACCTCACCAACT106850.10685No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)