FastQCFastQC Report
Tue 23 Feb 2021
in2197_6_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamein2197_6_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10000000
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTGTGAAATCTCGTA2511512.5115100000000004TruSeq Adapter, Index 12 (97% over 38bp)
TCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTGTGAAATCTCG861290.86129TruSeq Adapter, Index 12 (97% over 38bp)
CTGAGCCAACCTCCTGGCTGTCTATGCGACTCCACATCCTTTCCCACTTA229740.22973999999999997No Hit
CTGGGCTGTTTCCCTCTTGACAACGGACCTTATCACCCGCTGTCTGACTG170300.1703No Hit
CTCTCGTATTTAGCCTTGCCGGATGGTCCCGGCTGATTCAGACAGGATTT137760.13776000000000002No Hit
CTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTGTGAAATCTCG116260.11626TruSeq Adapter, Index 12 (97% over 38bp)
CTCTCGTATTTAGCCTTGCCGGATGGTCCCGGCCGATTCAGACAGGATTT107580.10758000000000001No Hit
CGTCGCCTTGGTGGGCCTTTACCCTCACCAACTAGCTAATCAGACGCAAG100560.10056No Hit
CAACGTCAGATATAGTTTGGTAAGCTGCCTTCGCAATCGGTGTTCTGTAT100170.10017No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)