FastQCFastQC Report
Tue 23 Feb 2021
in2197_7_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamein2197_7_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10000000
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTACACATCTCGTA2239222.23922TruSeq Adapter, Index 11 (97% over 40bp)
TCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTACACATCTCG685540.68554TruSeq Adapter, Index 11 (97% over 40bp)
CTGAGCCAACCTCCTGGCTGTCTATGCGACTCCACATCCTTTCCCACTTA231390.23138999999999998No Hit
CTGGGCTGTTTCCCTCTTGACAACGGACCTTATCACCCGCTGTCTGACTG171850.17185No Hit
CTCTCGTATTTAGCCTTGCCGGATGGTCCCGGCTGATTCAGACAGGATTT147040.14704No Hit
CGTCGCCTTGGTGGGCCTTTACCCTCACCAACTAGCTAATCAGACGCAAG119800.1198No Hit
CTCAGTCCGGCTACCGATCGCGGTCTTGGTGAGCCGTTACCTCACCAACT111480.11148No Hit
CTCTCGTATTTAGCCTTGCCGGATGGTCCCGGCCGATTCAGACAGGATTT107820.10782000000000001No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)