FastQCFastQC Report
Tue 23 Feb 2021
in2197_8_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamein2197_8_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10000000
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCTATCATCTCGTA2405412.40541TruSeq Adapter, Index 9 (97% over 37bp)
TCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCTATCATCTCG903010.9030099999999999TruSeq Adapter, Index 9 (97% over 37bp)
CTGAGCCAACCTCCTGGCTGTCTATGCGACTCCACATCCTTTCCCACTTA229080.22908No Hit
CTGGGCTGTTTCCCTCTTGACAACGGACCTTATCACCCGCTGTCTGACTG180520.18052No Hit
CTCTCGTATTTAGCCTTGCCGGATGGTCCCGGCTGATTCAGACAGGATTT137130.13713No Hit
CTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCTATCATCTCG115230.11523TruSeq Adapter, Index 9 (97% over 37bp)
CTCTCGTATTTAGCCTTGCCGGATGGTCCCGGCCGATTCAGACAGGATTT100030.10003No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)