FastQCFastQC Report
Tue 23 Feb 2021
in2197_9_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamein2197_9_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10000000
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAATTAACATCTCGTA2890102.8901TruSeq Adapter, Index 2 (97% over 37bp)
TCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAATTAACATCTCG863210.86321TruSeq Adapter, Index 2 (97% over 37bp)
CTGAGCCAACCTCCTGGCTGTCTATGCGACTCCACATCCTTTCCCACTTA169730.16973No Hit
CTGGGCTGTTTCCCTCTTGACAACGGACCTTATCACCCGCTGTCTGACTG168220.16822No Hit
CTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAATTAACATCTCG122890.12289TruSeq Adapter, Index 2 (97% over 37bp)
CTCTCGTATTTAGCCTTGCCGGATGGTCCCGGCTGATTCAGACAGGATTT110680.11068No Hit
CGTCGCCTTGGTGGGCCTTTACCCTCACCAACTAGCTAATCAGACGCAAG109060.10905999999999999No Hit
CTCTCGCTCACCTTAGGATTCTCTCCTCACCCATGTGTGTCCATTTTCGG100330.10032999999999999No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)